Construct: shRNA TRCN0000012025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008210.2-318s1c1
DNA Barcode:
CGCTATCAGAAGTCCACTGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H3f3a (15078)
Gene Description:
H3 histone, family 3A
Transcript:
RefSeq NM_008210.2 (NON-CURRENT)
Match location:
Position 318 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATCAGAAGTCCACTGAA
Hairpin Sequence:
5'-CCGG-CGCTATCAGAAGTCCACTGAA-CTCGAG-TTCAGTGGACTTCTGATAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATCAGAAGTCCACTGAACTCGAGTTCAGTGGACTTCTGATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATCAGAAGTCCACTGAACTCGAGTTCAGTGGACTTCTGATAGCG-3'

Other clones with same target sequence:

(none)