Construct: shRNA TRCN0000012028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_054044.1-4979s1c1
DNA Barcode:
GCCATGCTAATAATCTGACTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgra2 (78560)
Gene Description:
adhesion G protein-coupled receptor A2
Transcript:
RefSeq NM_054044.1 (NON-CURRENT)
Match location:
Position 4979 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATGCTAATAATCTGACTT
Hairpin Sequence:
5'-CCGG-GCCATGCTAATAATCTGACTT-CTCGAG-AAGTCAGATTATTAGCATGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATGCTAATAATCTGACTTCTCGAGAAGTCAGATTATTAGCATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATGCTAATAATCTGACTTCTCGAGAAGTCAGATTATTAGCATGGC-3'

Other clones with same target sequence:

  1. TRCN0000350174