Construct: shRNA TRCN0000012034

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026169.3-254s1c1
DNA Barcode:
CCGTGGAGAATTTGTCCTCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frmd8 (67457)
Gene Description:
FERM domain containing 8
Transcript:
RefSeq NM_026169.3 (NON-CURRENT)
Match location:
Position 254 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012034)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGGAGAATTTGTCCTCAA
Hairpin Sequence:
5'-CCGG-CCGTGGAGAATTTGTCCTCAA-CTCGAG-TTGAGGACAAATTCTCCACGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGGAGAATTTGTCCTCAACTCGAGTTGAGGACAAATTCTCCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGGAGAATTTGTCCTCAACTCGAGTTGAGGACAAATTCTCCACGG-3'

Other clones with same target sequence:

  1. TRCN0000350616