Construct: shRNA TRCN0000012071

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025282.1-460s1c1
DNA Barcode:
GCGTAACAGACAGGTGACTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mef2c (17260)
Gene Description:
myocyte enhancer factor 2C
Transcript:
RefSeq NM_025282.1 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012071)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTAACAGACAGGTGACTTT
Hairpin Sequence:
5'-CCGG-GCGTAACAGACAGGTGACTTT-CTCGAG-AAAGTCACCTGTCTGTTACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTAACAGACAGGTGACTTTCTCGAGAAAGTCACCTGTCTGTTACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTAACAGACAGGTGACTTTCTCGAGAAAGTCACCTGTCTGTTACGC-3'

Other clones with same target sequence:

(none)