Construct: shRNA TRCN0000012090

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008687.2-1496s1c1
DNA Barcode:
CCTTCCAGCTACTTCTCTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nfib (18028)
Gene Description:
nuclear factor I/B
Transcript:
RefSeq NM_008687.2 (NON-CURRENT)
Match location:
Position 1496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012090)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCCAGCTACTTCTCTCAT
Hairpin Sequence:
5'-CCGG-CCTTCCAGCTACTTCTCTCAT-CTCGAG-ATGAGAGAAGTAGCTGGAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCCAGCTACTTCTCTCATCTCGAGATGAGAGAAGTAGCTGGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCCAGCTACTTCTCTCATCTCGAGATGAGAGAAGTAGCTGGAAGG-3'

Other clones with same target sequence:

(none)