Construct: shRNA TRCN0000012112

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008666.1-1856s1c1
DNA Barcode:
CTCGGATATGATGAACCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Myt1l (17933)
Gene Description:
myelin transcription factor 1-like
Transcript:
RefSeq NM_008666.1 (NON-CURRENT)
Match location:
Position 1856 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012112)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGGATATGATGAACCTTAT
Hairpin Sequence:
5'-CCGG-CTCGGATATGATGAACCTTAT-CTCGAG-ATAAGGTTCATCATATCCGAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGGATATGATGAACCTTATCTCGAGATAAGGTTCATCATATCCGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGGATATGATGAACCTTATCTCGAGATAAGGTTCATCATATCCGAG-3'

Other clones with same target sequence:

(none)