Construct: shRNA TRCN0000012115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133948.2-367s1c1
DNA Barcode:
CCCACAAACAAACTACCCATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Psip1 (101739)
Gene Description:
PC4 and SFRS1 interacting protein 1
Transcript:
RefSeq NM_133948.2 (NON-CURRENT)
Match location:
Position 367 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCACAAACAAACTACCCATT
Hairpin Sequence:
5'-CCGG-CCCACAAACAAACTACCCATT-CTCGAG-AATGGGTAGTTTGTTTGTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCACAAACAAACTACCCATTCTCGAGAATGGGTAGTTTGTTTGTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCACAAACAAACTACCCATTCTCGAGAATGGGTAGTTTGTTTGTGGG-3'

Other clones with same target sequence:

  1. TRCN0000321213