Construct: shRNA TRCN0000012144

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019658.2-759s1c1
DNA Barcode:
CGGCATAATAAACTGAGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shoc2 (56392)
Gene Description:
soc-2 (suppressor of clear) homolog (C. elegans)
Transcript:
RefSeq NM_019658.2 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012144)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCATAATAAACTGAGAGAA
Hairpin Sequence:
5'-CCGG-CGGCATAATAAACTGAGAGAA-CTCGAG-TTCTCTCAGTTTATTATGCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCATAATAAACTGAGAGAACTCGAGTTCTCTCAGTTTATTATGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCATAATAAACTGAGAGAACTCGAGTTCTCTCAGTTTATTATGCCG-3'

Other clones with same target sequence:

(none)