Construct: shRNA TRCN0000012146

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019658.2-814s1c1
DNA Barcode:
CCACTCTCTATCTTCGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shoc2 (56392)
Gene Description:
soc-2 (suppressor of clear) homolog (C. elegans)
Transcript:
RefSeq NM_019658.2 (NON-CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012146)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCTCTATCTTCGCTTTA
Hairpin Sequence:
5'-CCGG-CCACTCTCTATCTTCGCTTTA-CTCGAG-TAAAGCGAAGATAGAGAGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCTCTATCTTCGCTTTACTCGAGTAAAGCGAAGATAGAGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCTCTATCTTCGCTTTACTCGAGTAAAGCGAAGATAGAGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000285055