Construct: shRNA TRCN0000012171

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025883.2-1470s1c1
DNA Barcode:
CGCTATAAAGTCATAGAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zdhhc6 (66980)
Gene Description:
zinc finger, DHHC domain containing 6
Transcript:
RefSeq NM_025883.2 (NON-CURRENT)
Match location:
Position 1470 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012171)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTATAAAGTCATAGAAGAT
Hairpin Sequence:
5'-CCGG-CGCTATAAAGTCATAGAAGAT-CTCGAG-ATCTTCTATGACTTTATAGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTATAAAGTCATAGAAGATCTCGAGATCTTCTATGACTTTATAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTATAAAGTCATAGAAGATCTCGAGATCTTCTATGACTTTATAGCG-3'

Other clones with same target sequence:

(none)