Construct: shRNA TRCN0000012173

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016862.2-1539s1c1
DNA Barcode:
GCGTGAGAATTGGCACAGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vti1a (53611)
Gene Description:
vesicle transport through interaction with t-SNAREs 1A
Transcript:
RefSeq NM_016862.2 (NON-CURRENT)
Match location:
Position 1539 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012173)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGAGAATTGGCACAGAAT
Hairpin Sequence:
5'-CCGG-GCGTGAGAATTGGCACAGAAT-CTCGAG-ATTCTGTGCCAATTCTCACGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGAGAATTGGCACAGAATCTCGAGATTCTGTGCCAATTCTCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGAGAATTGGCACAGAATCTCGAGATTCTGTGCCAATTCTCACGC-3'

Other clones with same target sequence:

(none)