Construct: shRNA TRCN0000012180

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009333.2-891s1c1
DNA Barcode:
GCTGACAGTCAACGCATCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tcf7l2 (21416)
Gene Description:
transcription factor 7 like 2, T cell specific, HMG box
Transcript:
RefSeq NM_009333.2 (NON-CURRENT)
Match location:
Position 891 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012180)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACAGTCAACGCATCTAT
Hairpin Sequence:
5'-CCGG-GCTGACAGTCAACGCATCTAT-CTCGAG-ATAGATGCGTTGACTGTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACAGTCAACGCATCTATCTCGAGATAGATGCGTTGACTGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACAGTCAACGCATCTATCTCGAGATAGATGCGTTGACTGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000321290