Construct: shRNA TRCN0000012193

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007611.1-1128s1c1
DNA Barcode:
CGTTCGTTGATGAATCCAGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Casp7 (12369)
Gene Description:
caspase 7
Transcript:
RefSeq NM_007611.1 (NON-CURRENT)
Match location:
Position 1128 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012193)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTTCGTTGATGAATCCAGTT
Hairpin Sequence:
5'-CCGG-CGTTCGTTGATGAATCCAGTT-CTCGAG-AACTGGATTCATCAACGAACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTTCGTTGATGAATCCAGTTCTCGAGAACTGGATTCATCAACGAACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTTCGTTGATGAATCCAGTTCTCGAGAACTGGATTCATCAACGAACG-3'

Other clones with same target sequence:

  1. TRCN0000297287