Construct: shRNA TRCN0000012249

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015733.3-352s1c1
DNA Barcode:
CGCGACATGATCGAGGATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Casp9 (12371)
Gene Description:
caspase 9
Transcript:
RefSeq NM_015733.3 (NON-CURRENT)
Match location:
Position 352 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012249)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGACATGATCGAGGATATT
Hairpin Sequence:
5'-CCGG-CGCGACATGATCGAGGATATT-CTCGAG-AATATCCTCGATCATGTCGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGACATGATCGAGGATATTCTCGAGAATATCCTCGATCATGTCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGACATGATCGAGGATATTCTCGAGAATATCCTCGATCATGTCGCG-3'

Other clones with same target sequence:

  1. TRCN0000321027