Construct: shRNA TRCN0000012313

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019752.2-1564s1c1
DNA Barcode:
CCTCCTTGCTTTCCTAGCCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Htra2 (64704)
Gene Description:
HtrA serine peptidase 2
Transcript:
RefSeq NM_019752.2 (NON-CURRENT)
Match location:
Position 1564 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012313)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTTGCTTTCCTAGCCTA
Hairpin Sequence:
5'-CCGG-CCTCCTTGCTTTCCTAGCCTA-CTCGAG-TAGGCTAGGAAAGCAAGGAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTTGCTTTCCTAGCCTACTCGAGTAGGCTAGGAAAGCAAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTTGCTTTCCTAGCCTACTCGAGTAGGCTAGGAAAGCAAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000273062