Construct: shRNA TRCN0000012322

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011610.1-814s1c1
DNA Barcode:
GCCAAACTCCAAGCATCCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnfrsf1b (21938)
Gene Description:
tumor necrosis factor receptor superfamily, member 1b
Transcript:
RefSeq NM_011610.1 (NON-CURRENT)
Match location:
Position 814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012322)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAACTCCAAGCATCCTTA
Hairpin Sequence:
5'-CCGG-GCCAAACTCCAAGCATCCTTA-CTCGAG-TAAGGATGCTTGGAGTTTGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAACTCCAAGCATCCTTACTCGAGTAAGGATGCTTGGAGTTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAACTCCAAGCATCCTTACTCGAGTAAGGATGCTTGGAGTTTGGC-3'

Other clones with same target sequence:

(none)