Construct: shRNA TRCN0000012339
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008362.1-1731s1c1
- DNA Barcode:
- CCAGATTCTATTCAGTTCATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Il1r1 (16177)
- Gene Description:
- interleukin 1 receptor, type I
- Transcript:
- RefSeq NM_008362.1 (NON-CURRENT)
- Match location:
- Position 1731 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000012339)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCAGATTCTATTCAGTTCATT
- Hairpin Sequence:
-
5'-CCGG-CCAGATTCTATTCAGTTCATT-CTCGAG-AATGAACTGAATAGAATCTGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCAGATTCTATTCAGTTCATTCTCGAGAATGAACTGAATAGAATCTGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCAGATTCTATTCAGTTCATTCTCGAGAATGAACTGAATAGAATCTGG-3'
Other clones with same target sequence:
-
TRCN0000344990
