Construct: shRNA TRCN0000012340

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008362.1-389s1c1
DNA Barcode:
CGACACCATAATTTGGTACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Il1r1 (16177)
Gene Description:
interleukin 1 receptor, type I
Transcript:
RefSeq NM_008362.1 (NON-CURRENT)
Match location:
Position 389 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012340)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACACCATAATTTGGTACAA
Hairpin Sequence:
5'-CCGG-CGACACCATAATTTGGTACAA-CTCGAG-TTGTACCAAATTATGGTGTCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACACCATAATTTGGTACAACTCGAGTTGTACCAAATTATGGTGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACACCATAATTTGGTACAACTCGAGTTGTACCAAATTATGGTGTCG-3'

Other clones with same target sequence:

  1. TRCN0000344988