Construct: shRNA TRCN0000012351

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007700.1-1956s1c1
DNA Barcode:
GCTGACAATACTGTCATGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chuk (12675)
Gene Description:
conserved helix-loop-helix ubiquitous kinase
Transcript:
RefSeq NM_007700.1 (NON-CURRENT)
Match location:
Position 1956 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012351)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGACAATACTGTCATGTTT
Hairpin Sequence:
5'-CCGG-GCTGACAATACTGTCATGTTT-CTCGAG-AAACATGACAGTATTGTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGACAATACTGTCATGTTTCTCGAGAAACATGACAGTATTGTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGACAATACTGTCATGTTTCTCGAGAAACATGACAGTATTGTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000280139