Construct: shRNA TRCN0000012351
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_007700.1-1956s1c1
- DNA Barcode:
- GCTGACAATACTGTCATGTTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Chuk (12675)
- Gene Description:
- conserved helix-loop-helix ubiquitous kinase
- Transcript:
- RefSeq NM_007700.1 (NON-CURRENT)
- Match location:
- Position 1956 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000012351)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCTGACAATACTGTCATGTTT
- Hairpin Sequence:
-
5'-CCGG-GCTGACAATACTGTCATGTTT-CTCGAG-AAACATGACAGTATTGTCAGC-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCTGACAATACTGTCATGTTTCTCGAGAAACATGACAGTATTGTCAGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCTGACAATACTGTCATGTTTCTCGAGAAACATGACAGTATTGTCAGC-3'
Other clones with same target sequence:
-
TRCN0000280139
