Construct: shRNA TRCN0000012402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010292.3-1296s1c1
DNA Barcode:
CAACTGCGAAATCACCTTCAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gck (103988)
Gene Description:
glucokinase
Transcript:
RefSeq NM_010292.3 (NON-CURRENT)
Match location:
Position 1296 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTGCGAAATCACCTTCAT
Hairpin Sequence:
5'-CCGG-CAACTGCGAAATCACCTTCAT-CTCGAG-ATGAAGGTGATTTCGCAGTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTGCGAAATCACCTTCATCTCGAGATGAAGGTGATTTCGCAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTGCGAAATCACCTTCATCTCGAGATGAAGGTGATTTCGCAGTTG-3'

Other clones with same target sequence:

(none)