Construct: shRNA TRCN0000012417

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017374.2-870s1c1
DNA Barcode:
GCCCAATGTGTGATCTCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2cb (19053)
Gene Description:
protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Transcript:
RefSeq NM_017374.2 (NON-CURRENT)
Match location:
Position 870 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012417)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCAATGTGTGATCTCTTAT
Hairpin Sequence:
5'-CCGG-GCCCAATGTGTGATCTCTTAT-CTCGAG-ATAAGAGATCACACATTGGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCAATGTGTGATCTCTTATCTCGAGATAAGAGATCACACATTGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCAATGTGTGATCTCTTATCTCGAGATAAGAGATCACACATTGGGC-3'

Other clones with same target sequence:

  1. TRCN0000294513