Construct: shRNA TRCN0000012430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009722.1-2015s1c1
DNA Barcode:
CCAGGATTGAAGTAGCCTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp2a2 (11938)
Gene Description:
ATPase, Ca++ transporting, cardiac muscle, slow twitch 2
Transcript:
RefSeq NM_009722.1 (NON-CURRENT)
Match location:
Position 2015 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGGATTGAAGTAGCCTCTT
Hairpin Sequence:
5'-CCGG-CCAGGATTGAAGTAGCCTCTT-CTCGAG-AAGAGGCTACTTCAATCCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGGATTGAAGTAGCCTCTTCTCGAGAAGAGGCTACTTCAATCCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGGATTGAAGTAGCCTCTTCTCGAGAAGAGGCTACTTCAATCCTGG-3'

Other clones with same target sequence:

(none)