Construct: shRNA TRCN0000012470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009792.1-495s1c1
DNA Barcode:
CGCAAACAGGAAATTATCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk2a (12322)
Gene Description:
calcium/calmodulin-dependent protein kinase II alpha
Transcript:
RefSeq NM_009792.1 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAAACAGGAAATTATCAAA
Hairpin Sequence:
5'-CCGG-CGCAAACAGGAAATTATCAAA-CTCGAG-TTTGATAATTTCCTGTTTGCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAAACAGGAAATTATCAAACTCGAGTTTGATAATTTCCTGTTTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAAACAGGAAATTATCAAACTCGAGTTTGATAATTTCCTGTTTGCG-3'

Other clones with same target sequence:

(none)