Construct: shRNA TRCN0000012475

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007595.2-409s1c1
DNA Barcode:
CCTGCTGAAGCATTCCAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk2b (12323)
Gene Description:
calcium/calmodulin-dependent protein kinase II, beta
Transcript:
RefSeq NM_007595.2 (NON-CURRENT)
Match location:
Position 409 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012475)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTGAAGCATTCCAACAT
Hairpin Sequence:
5'-CCGG-CCTGCTGAAGCATTCCAACAT-CTCGAG-ATGTTGGAATGCTTCAGCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTGAAGCATTCCAACATCTCGAGATGTTGGAATGCTTCAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTGAAGCATTCCAACATCTCGAGATGTTGGAATGCTTCAGCAGG-3'

Other clones with same target sequence:

  1. TRCN0000319747