Construct: shRNA TRCN0000012482

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023813.2-1254s1c1
DNA Barcode:
CCTGAAGCATTGGGCAACTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Camk2d (108058)
Gene Description:
calcium/calmodulin-dependent protein kinase II, delta
Transcript:
RefSeq NM_023813.2 (NON-CURRENT)
Match location:
Position 1254 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012482)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGCATTGGGCAACTTA
Hairpin Sequence:
5'-CCGG-CCTGAAGCATTGGGCAACTTA-CTCGAG-TAAGTTGCCCAATGCTTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGCATTGGGCAACTTACTCGAGTAAGTTGCCCAATGCTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGCATTGGGCAACTTACTCGAGTAAGTTGCCCAATGCTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000345113