Construct: shRNA TRCN0000012484

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024459.1-227s1c1
DNA Barcode:
CCTTTAGTACAGCGGGTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp3r1 (19058)
Gene Description:
protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I)
Transcript:
RefSeq NM_024459.1 (NON-CURRENT)
Match location:
Position 227 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012484)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTTAGTACAGCGGGTAATA
Hairpin Sequence:
5'-CCGG-CCTTTAGTACAGCGGGTAATA-CTCGAG-TATTACCCGCTGTACTAAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTTAGTACAGCGGGTAATACTCGAGTATTACCCGCTGTACTAAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTTAGTACAGCGGGTAATACTCGAGTATTACCCGCTGTACTAAAGG-3'

Other clones with same target sequence:

  1. TRCN0000280152