Construct: shRNA TRCN0000012532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010438.1-2095s1c1
DNA Barcode:
GAGCCTAGATTGCGGAATCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hk1 (15275)
Gene Description:
hexokinase 1
Transcript:
RefSeq NM_010438.1 (NON-CURRENT)
Match location:
Position 2095 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCCTAGATTGCGGAATCTT
Hairpin Sequence:
5'-CCGG-GAGCCTAGATTGCGGAATCTT-CTCGAG-AAGATTCCGCAATCTAGGCTC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCCTAGATTGCGGAATCTTCTCGAGAAGATTCCGCAATCTAGGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCCTAGATTGCGGAATCTTCTCGAGAAGATTCCGCAATCTAGGCTC-3'

Other clones with same target sequence:

  1. TRCN0000297387