Construct: shRNA TRCN0000012542

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008826.2-1707s1c1
DNA Barcode:
GCTGCAATGGAGAGTTGTGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pfkl (18641)
Gene Description:
phosphofructokinase, liver, B-type
Transcript:
RefSeq NM_008826.2 (NON-CURRENT)
Match location:
Position 1707 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012542)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCAATGGAGAGTTGTGAT
Hairpin Sequence:
5'-CCGG-GCTGCAATGGAGAGTTGTGAT-CTCGAG-ATCACAACTCTCCATTGCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCAATGGAGAGTTGTGATCTCGAGATCACAACTCTCCATTGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCAATGGAGAGTTGTGATCTCGAGATCACAACTCTCCATTGCAGC-3'

Other clones with same target sequence:

(none)