Construct: shRNA TRCN0000012575

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008783.1-796s1c1
DNA Barcode:
GCCTGCCTTGTTTAATGTGTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pbx1 (18514)
Gene Description:
pre B cell leukemia homeobox 1
Transcript:
RefSeq NM_008783.1 (NON-CURRENT)
Match location:
Position 796 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012575)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGCCTTGTTTAATGTGTT
Hairpin Sequence:
5'-CCGG-GCCTGCCTTGTTTAATGTGTT-CTCGAG-AACACATTAAACAAGGCAGGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGCCTTGTTTAATGTGTTCTCGAGAACACATTAAACAAGGCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGCCTTGTTTAATGTGTTCTCGAGAACACATTAAACAAGGCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000321049