Construct: shRNA TRCN0000012583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016700.2-1377s1c1
DNA Barcode:
CGTCTGTCAATGACATGTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mapk8 (26419)
Gene Description:
mitogen-activated protein kinase 8
Transcript:
RefSeq NM_016700.2 (NON-CURRENT)
Match location:
Position 1377 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTCTGTCAATGACATGTCTT
Hairpin Sequence:
5'-CCGG-CGTCTGTCAATGACATGTCTT-CTCGAG-AAGACATGTCATTGACAGACG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTCTGTCAATGACATGTCTTCTCGAGAAGACATGTCATTGACAGACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTCTGTCAATGACATGTCTTCTCGAGAAGACATGTCATTGACAGACG-3'

Other clones with same target sequence:

(none)