Construct: shRNA TRCN0000012620

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012016.1-903s1c1
DNA Barcode:
GCTGGGTTCTATGTCTCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ern2 (26918)
Gene Description:
endoplasmic reticulum (ER) to nucleus signalling 2
Transcript:
RefSeq NM_012016.1 (NON-CURRENT)
Match location:
Position 903 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012620)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGTTCTATGTCTCTAAA
Hairpin Sequence:
5'-CCGG-GCTGGGTTCTATGTCTCTAAA-CTCGAG-TTTAGAGACATAGAACCCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGTTCTATGTCTCTAAACTCGAGTTTAGAGACATAGAACCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGTTCTATGTCTCTAAACTCGAGTTTAGAGACATAGAACCCAGC-3'

Other clones with same target sequence:

(none)