Construct: shRNA TRCN0000012662

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011234.2-285s1c1
DNA Barcode:
CCACAGCCTATTTCACGGTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rad51 (19361)
Gene Description:
RAD51 recombinase
Transcript:
RefSeq NM_011234.2 (NON-CURRENT)
Match location:
Position 285 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012662)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGCCTATTTCACGGTTA
Hairpin Sequence:
5'-CCGG-CCACAGCCTATTTCACGGTTA-CTCGAG-TAACCGTGAAATAGGCTGTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGCCTATTTCACGGTTACTCGAGTAACCGTGAAATAGGCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGCCTATTTCACGGTTACTCGAGTAACCGTGAAATAGGCTGTGG-3'

Other clones with same target sequence:

  1. TRCN0000319679