Construct: shRNA TRCN0000012668

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013752.2-2380s1c1
DNA Barcode:
AGCTGTAGAAACACATTTCTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nbn (27354)
Gene Description:
nibrin
Transcript:
RefSeq NM_013752.2 (NON-CURRENT)
Match location:
Position 2380 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012668)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGTAGAAACACATTTCTA
Hairpin Sequence:
5'-CCGG-AGCTGTAGAAACACATTTCTA-CTCGAG-TAGAAATGTGTTTCTACAGCT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGTAGAAACACATTTCTACTCGAGTAGAAATGTGTTTCTACAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGTAGAAACACATTTCTACTCGAGTAGAAATGTGTTTCTACAGCT-3'

Other clones with same target sequence:

  1. TRCN0000273047