Construct: shRNA TRCN0000012696

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008915.1-1322s1c1
DNA Barcode:
CGGATGAAGAAATGAACGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp3cc (19057)
Gene Description:
protein phosphatase 3, catalytic subunit, gamma isoform
Transcript:
RefSeq NM_008915.1 (NON-CURRENT)
Match location:
Position 1322 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012696)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATGAAGAAATGAACGTAA
Hairpin Sequence:
5'-CCGG-CGGATGAAGAAATGAACGTAA-CTCGAG-TTACGTTCATTTCTTCATCCG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATGAAGAAATGAACGTAACTCGAGTTACGTTCATTTCTTCATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATGAAGAAATGAACGTAACTCGAGTTACGTTCATTTCTTCATCCG-3'

Other clones with same target sequence:

(none)