Construct: shRNA TRCN0000012723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_148699.3-4387s1c1
DNA Barcode:
CCGGGAATGAAGTCAAGGTTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crebbp (12914)
Gene Description:
CREB binding protein
Transcript:
RefSeq XM_148699.3 (NON-CURRENT)
Match location:
Position 4387 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGGGAATGAAGTCAAGGTTT
Hairpin Sequence:
5'-CCGG-CCGGGAATGAAGTCAAGGTTT-CTCGAG-AAACCTTGACTTCATTCCCGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGGGAATGAAGTCAAGGTTTCTCGAGAAACCTTGACTTCATTCCCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGGGAATGAAGTCAAGGTTTCTCGAGAAACCTTGACTTCATTCCCGG-3'

Other clones with same target sequence:

(none)