Construct: shRNA TRCN0000012740

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008683.1-184s1c1
DNA Barcode:
GCGGCTCATCTACAGTGGCAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nedd8 (18002)
Gene Description:
neural precursor cell expressed, developmentally down-regulated gene 8
Transcript:
RefSeq NM_008683.1 (NON-CURRENT)
Match location:
Position 184 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012740)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGCTCATCTACAGTGGCAA
Hairpin Sequence:
5'-CCGG-GCGGCTCATCTACAGTGGCAA-CTCGAG-TTGCCACTGTAGATGAGCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGCTCATCTACAGTGGCAACTCGAGTTGCCACTGTAGATGAGCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGCTCATCTACAGTGGCAACTCGAGTTGCCACTGTAGATGAGCCGC-3'

Other clones with same target sequence:

(none)