Construct: shRNA TRCN0000012836

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010752.1-120s1c1
DNA Barcode:
CAACTTCATTTCTCAGCGAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mad1l1 (17120)
Gene Description:
MAD1 mitotic arrest deficient 1-like 1
Transcript:
RefSeq NM_010752.1 (NON-CURRENT)
Match location:
Position 120 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012836)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACTTCATTTCTCAGCGAAT
Hairpin Sequence:
5'-CCGG-CAACTTCATTTCTCAGCGAAT-CTCGAG-ATTCGCTGAGAAATGAAGTTG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACTTCATTTCTCAGCGAATCTCGAGATTCGCTGAGAAATGAAGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACTTCATTTCTCAGCGAATCTCGAGATTCGCTGAGAAATGAAGTTG-3'

Other clones with same target sequence:

  1. TRCN0000273055