Construct: shRNA TRCN0000012850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009772.1-448s1c1
DNA Barcode:
GCTGAACCTAAAGAACTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bub1 (12235)
Gene Description:
BUB1, mitotic checkpoint serine/threonine kinase
Transcript:
RefSeq NM_009772.1 (NON-CURRENT)
Match location:
Position 448 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000012850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGAACCTAAAGAACTACTA
Hairpin Sequence:
5'-CCGG-GCTGAACCTAAAGAACTACTA-CTCGAG-TAGTAGTTCTTTAGGTTCAGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGAACCTAAAGAACTACTACTCGAGTAGTAGTTCTTTAGGTTCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGAACCTAAAGAACTACTACTCGAGTAGTAGTTCTTTAGGTTCAGC-3'

Other clones with same target sequence:

  1. TRCN0000345051