Construct: shRNA TRCN0000013136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004641.1-681s1c1
DNA Barcode:
CCATGTAACATGCGCTCAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MLLT10 (8028)
Gene Description:
MLLT10 histone lysine methyltransferase DOT1L cofactor
Transcript:
RefSeq NM_004641.1 (NON-CURRENT)
Match location:
Position 681 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTAACATGCGCTCAGTT
Hairpin Sequence:
5'-CCGG-CCATGTAACATGCGCTCAGTT-CTCGAG-AACTGAGCGCATGTTACATGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTAACATGCGCTCAGTTCTCGAGAACTGAGCGCATGTTACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTAACATGCGCTCAGTTCTCGAGAACTGAGCGCATGTTACATGG-3'

Other clones with same target sequence:

(none)