Construct: shRNA TRCN0000013181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004188.2-286s1c1
DNA Barcode:
CCTTAGCACTCTATTCCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFI1B (8328)
Gene Description:
growth factor independent 1B transcriptional repressor
Transcript:
RefSeq NM_004188.2 (NON-CURRENT)
Match location:
Position 286 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTAGCACTCTATTCCCAAA
Hairpin Sequence:
5'-CCGG-CCTTAGCACTCTATTCCCAAA-CTCGAG-TTTGGGAATAGAGTGCTAAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTAGCACTCTATTCCCAAACTCGAGTTTGGGAATAGAGTGCTAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTAGCACTCTATTCCCAAACTCGAGTTTGGGAATAGAGTGCTAAGG-3'

Other clones with same target sequence:

(none)