Construct: shRNA TRCN0000013196

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003593.2-1662s1c1
DNA Barcode:
CTGTGGGAACAGTTGAAGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FOXN1 (8456)
Gene Description:
forkhead box N1
Transcript:
RefSeq NM_003593.2 (CURRENT)
Match location:
Position 1662 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013196)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGTGGGAACAGTTGAAGGAT
Hairpin Sequence:
5'-CCGG-CTGTGGGAACAGTTGAAGGAT-CTCGAG-ATCCTTCAACTGTTCCCACAG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGTGGGAACAGTTGAAGGATCTCGAGATCCTTCAACTGTTCCCACAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGTGGGAACAGTTGAAGGATCTCGAGATCCTTCAACTGTTCCCACAG-3'

Other clones with same target sequence:

(none)