Construct: shRNA TRCN0000013208

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003599.1-708s1c1
DNA Barcode:
CCCAATGTTGTCGCAATGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SUPT3H (8464)
Gene Description:
SPT3 homolog, SAGA and STAGA complex component
Transcript:
RefSeq NM_003599.1 (NON-CURRENT)
Match location:
Position 708 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013208)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATGTTGTCGCAATGGAA
Hairpin Sequence:
5'-CCGG-CCCAATGTTGTCGCAATGGAA-CTCGAG-TTCCATTGCGACAACATTGGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATGTTGTCGCAATGGAACTCGAGTTCCATTGCGACAACATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATGTTGTCGCAATGGAACTCGAGTTCCATTGCGACAACATTGGG-3'

Other clones with same target sequence:

(none)