Construct: shRNA TRCN0000013271

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003791.2-622s1c1
DNA Barcode:
CCTGACTTTGAAGGTGGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MBTPS1 (8720)
Gene Description:
membrane bound transcription factor peptidase, site 1
Transcript:
RefSeq NM_003791.2 (NON-CURRENT)
Match location:
Position 622 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013271)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGACTTTGAAGGTGGAATT
Hairpin Sequence:
5'-CCGG-CCTGACTTTGAAGGTGGAATT-CTCGAG-AATTCCACCTTCAAAGTCAGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGACTTTGAAGGTGGAATTCTCGAGAATTCCACCTTCAAAGTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGACTTTGAAGGTGGAATTCTCGAGAATTCCACCTTCAAAGTCAGG-3'

Other clones with same target sequence:

(none)