Construct: shRNA TRCN0000013291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006022.2-383s1c1
DNA Barcode:
GCAAGCTATGGATCTAGTGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TSC22D1 (8848)
Gene Description:
TSC22 domain family member 1
Transcript:
RefSeq NM_006022.2 (NON-CURRENT)
Match location:
Position 383 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGCTATGGATCTAGTGAA
Hairpin Sequence:
5'-CCGG-GCAAGCTATGGATCTAGTGAA-CTCGAG-TTCACTAGATCCATAGCTTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGCTATGGATCTAGTGAACTCGAGTTCACTAGATCCATAGCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGCTATGGATCTAGTGAACTCGAGTTCACTAGATCCATAGCTTGC-3'

Other clones with same target sequence:

(none)