Construct: shRNA TRCN0000013343
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003972.1-5966s1c1
- DNA Barcode:
- CCTCCTATCTTTAGTCACTTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BTAF1 (9044)
- Gene Description:
- B-TFIID TATA-box binding protein associated factor 1
- Transcript:
- RefSeq NM_003972.1 (NON-CURRENT)
- Match location:
- Position 5966 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000013343)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CCTCCTATCTTTAGTCACTTT
- Hairpin Sequence:
-
5'-CCGG-CCTCCTATCTTTAGTCACTTT-CTCGAG-AAAGTGACTAAAGATAGGAGG-TTTTT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCCTCCTATCTTTAGTCACTTTCTCGAGAAAGTGACTAAAGATAGGAGGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACCTCCTATCTTTAGTCACTTTCTCGAGAAAGTGACTAAAGATAGGAGG-3'
Other clones with same target sequence:
-
TRCN0000274320
