Construct: shRNA TRCN0000013359

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004689.2-433s1c1
DNA Barcode:
GCGGGAGGATTTCTTCTTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTA1 (9112)
Gene Description:
metastasis associated 1
Transcript:
RefSeq NM_004689.2 (NON-CURRENT)
Match location:
Position 433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013359)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGGGAGGATTTCTTCTTCTA
Hairpin Sequence:
5'-CCGG-GCGGGAGGATTTCTTCTTCTA-CTCGAG-TAGAAGAAGAAATCCTCCCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGGGAGGATTTCTTCTTCTACTCGAGTAGAAGAAGAAATCCTCCCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGGGAGGATTTCTTCTTCTACTCGAGTAGAAGAAGAAATCCTCCCGC-3'

Other clones with same target sequence:

(none)