Construct: shRNA TRCN0000013362

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004689.2-1257s1c1
DNA Barcode:
GCGCATCTTGTTGGACATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTA1 (9112)
Gene Description:
metastasis associated 1
Transcript:
RefSeq NM_004689.2 (NON-CURRENT)
Match location:
Position 1257 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013362)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCATCTTGTTGGACATATT
Hairpin Sequence:
5'-CCGG-GCGCATCTTGTTGGACATATT-CTCGAG-AATATGTCCAACAAGATGCGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCATCTTGTTGGACATATTCTCGAGAATATGTCCAACAAGATGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCATCTTGTTGGACATATTCTCGAGAATATGTCCAACAAGATGCGC-3'

Other clones with same target sequence:

(none)