Construct: shRNA TRCN0000013465

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004865.2-466s1c1
DNA Barcode:
ACCTAGAATTACAGCTACAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBPL1 (9519)
Gene Description:
TATA-box binding protein like 1
Transcript:
RefSeq NM_004865.2 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013465)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTAGAATTACAGCTACAAT
Hairpin Sequence:
5'-CCGG-ACCTAGAATTACAGCTACAAT-CTCGAG-ATTGTAGCTGTAATTCTAGGT-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTAGAATTACAGCTACAATCTCGAGATTGTAGCTGTAATTCTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTAGAATTACAGCTACAATCTCGAGATTGTAGCTGTAATTCTAGGT-3'

Other clones with same target sequence:

  1. TRCN0000274264