Construct: shRNA TRCN0000013505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014724.2-862s1c1
DNA Barcode:
CCAGAGAAGATAAACAACCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSCAN12 (9753)
Gene Description:
zinc finger and SCAN domain containing 12
Transcript:
RefSeq NM_014724.2 (NON-CURRENT)
Match location:
Position 862 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAGAAGATAAACAACCTA
Hairpin Sequence:
5'-CCGG-CCAGAGAAGATAAACAACCTA-CTCGAG-TAGGTTGTTTATCTTCTCTGG-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAGAAGATAAACAACCTACTCGAGTAGGTTGTTTATCTTCTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAGAAGATAAACAACCTACTCGAGTAGGTTGTTTATCTTCTCTGG-3'

Other clones with same target sequence:

(none)