Construct: shRNA TRCN0000013528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014795.2-4958s1c1
DNA Barcode:
GCAGTTCCTTAGTTTACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZEB2 (9839)
Gene Description:
zinc finger E-box binding homeobox 2
Transcript:
RefSeq NM_014795.2 (NON-CURRENT)
Match location:
Position 4958 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000013528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTTCCTTAGTTTACATAT
Hairpin Sequence:
5'-CCGG-GCAGTTCCTTAGTTTACATAT-CTCGAG-ATATGTAAACTAAGGAACTGC-TTTTT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTTCCTTAGTTTACATATCTCGAGATATGTAAACTAAGGAACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTTCCTTAGTTTACATATCTCGAGATATGTAAACTAAGGAACTGC-3'

Other clones with same target sequence:

(none)